(Important note: STTM is an information-only site based on what many patients have reported or learned in their treatment. Please work with your doctor. This is not meant to replace that relationship or guidance, and you agree to that by reading this website. See the Disclaimer.)

Did you know that STTM was the first to find out and report all the below? This is where it all began years ago. 

i.e. when thyroid patients started looking at each others lab results years ago in a group started by Janie Bowthorpe, we observed repeatedly that it’s WHERE the results fall (in any range given) that had meaning…not simply that they “fall in the normal range”!

Please work with your doctor in all this.


a) For most of the below, we learned from our more progressive doctors that you’ll need to be off what you are testing for a minimum of 12 hours, i.e. take nothing the morning of the test.  
b) For iron, we learned to be off for 5 days based on information from the Iron Institute, i.e. to see what we are “holding onto”.
c) For saliva, we learned to be off any cortisol-containing or cortisol-changing supplement for up to two weeks, “if possible” and in working with your doctor. Some things we may not be able to get off of, though.

**NOTE: All the below is copyrighted by Stop the Thyroid Madness. It is not permissible to post it elsewhere without seeking permission from Janie Bowthorpe. 


24-HOUR CORTISOL SALIVA: This is an at-home test to evaluate your circadian and cellular cortisol levels at key times during a 24 hour period. It’s more accurate than blood cortisol, we saw repeatedly, since the latter measures both bound and unbound cortisol, not cellular levels like saliva, and thus blood can give a false idea of what is really going on.

We noted repeatedly that those will healthy adrenal function will have the following saliva results:

8 am: at the literal tip top of the range
11 am-noon: in the upper quarter
4-5 pm: mid-range
11 pm to midnight: at the very bottom (literally).

For accuracy, patients learned they needed to be off any adrenal support supplement, any medication containing cortisol, any herbs that support adrenal function, or zinc or licorice root for at least two weeks prior to testing…if possible  (It may not be possible for some conditions, though, to be off certain meds or support–work with your doctor!) Avoid food when spitting into vials.  If you wear dentures, remove them to prevent denture adhesives from tainting the spit, says the facilities.

Note: we learned the hard way that it’s impossible to know what is going on if you use a lab facility like Quest that has the range as “less than” or “more than” a number. (i.e. < or >).

ALDOSTERONE: Measures the adrenal hormone aldosterone which helps regulate levels of sodium and potassium in your body–i.e. it helps you retain needed salt, which in turn helps control your blood pressure, the distribution of fluids in the body, and the balance of electrolytes in your blood. We’ve noticed that if you are “around” mid-range or below, which is often 4.0 – 31.0 ng/dl (or 0 – 28, for example) along with symptoms of low aldosterone , there is reason to be suspicious that your adrenals aren’t producing enough, since healthy adrenals will generally put you higher than mid-range.This is best tested in the morning and with no salt intake for 24 hours. Women need to do it in the first week after their period, since rising progesterone can also raise your aldosterone. Testing should not be done with severe illness (aldosterone falls in response to severe illness), during periods of intense stress (aldosterone rises), or right after strenuous exercise (aldosterone rises). Being pregnant can result in doubled amounts of aldosterone.  

Are you a menstruating female? It’s strongly recommended to test your aldosterone in the first week of your menstrual cycle (i.e. the week you start bleeding), or up until the beginning of the week after–this is  when progesterone is at its lowest.  Progesterone can drive your aldosterone up falsely, and progesterone begins to rise at the end of the second week of your cycle.   

DHEA: Measures the mother of all steroid & sex hormones. Usually measured in conjunction with the 24-hour adrenal cortisol saliva test.  See above. It’s hard to know exactly where anyone’s DHEA “should” be. We know by what we read that DHEA begins a decline generally in your 30’s, and can be much by the time of the 70’s. But here’s something interesting we’ve noticed: in the presence of a low cortisol problem, the DHEA will rise towards the top, attempting to compensate for the low cortisol. But as the low cortisol continues, DHEA falls fairly low in the range.

ACTH STIM (not needed for most, we’ve noted, unless there is suspicion of a serious adrenal problem): The ACTH Stimulation test, also called the Cosyntropin test, measures the ability of your adrenals to be stimulated by the ACTH, a pituitary hormone, and is used to diagnose Addison’s or Cushing’s disease, as well as hypopituitary.  Usually done in an out-patient setting and takes only a few hours.  A synthetic ACTH is injected into your arm and the response of your plasma cortisol levels are measured. You’ll need to fast, and the test is usually done in the morning. You cannot be on any cortisol medications or supplements.  An ACTH plasma test is often done at the same time to measure the amount of ACTH being secreted by the pituitary gland. Your cortisol levels will double if your adrenals are not diseased. The ACTH has not been found to be a good test for the kind of adrenal fatigue manifested by thyroid patients, which is sluggishness, not disease.


FREE T3: T3 is the active thyroid  hormone. Free in front of the T3 means you are measuring what is available and unbound.  Those on an optimal amount of desiccated thyroid, with no lingering hypothyroid symptoms and in the presence of healthy adrenals, tend to have a free T3 towards the upper part of the range, patients have repeatedly noticed and reported. If you are on desiccated thyroid (especially if lower than 3 grains) and find yourself with the free T3 high or above range in the presence of continuing hypothyroid symptoms, or even hyper-like symptoms (anxiety, shakiness), patients have noted it can be a clue you have adrenal fatigue, aka low cortisol.  If not on thyroid medication: 1) If your free T3 is high, you could have Hashimoto’s disease, which will need the two antibodies tests to discern it, or Graves disease, which needs the TSI test. 2) if your free T3 is mid-range or lower, and in the presence of hypothyroid symptoms, you may have hypothyroidism, no matter how low the TSH. Patients find it’s not a good idea to take any T3-containing product on the morning of a test. Work with your doctor.

FREE T4: T4 is the thyroid storage hormone. Free in front of the T4 means you are measuring what is available and unbound.  Generally, those on an optimal amount of desiccated thyroid will have a free T4 around mid-range when their free T3 is at the top and in the presence of healthy adrenals. If you have low FT4 and a mid-range or slightly higher FT3, it usually means the T4 is converting like mad to give you the T3 you do have, which means hypo.

REVERSE T3: The body produces the benign RT3 naturally to rid itself of excess of T4 at any time, but in some cases, such as high or low cortisol, or having inadequate levels of iron, it’s made in excess and that excess clogs your cell receptors from receiving regular T3. (See page 162-163 in the STTM book for further details.) Two ways to figure this one out, patient experiences have discovered:

1) To look at our ratio between the free T3 and RT3 by doing them at the same time. We’ve learned and observed that those who don’t have a serious RT3 problem have a ratio of over 20, i.e. FT3 is 20 times or higher than the RT3.
2) To look at an individual RT3 number. For ranges which start at 8 or 9, seeing a result start to move over 11 is a sign that RT3 is going up towards being a problem. In Australia or other places, here are ranges and what you want to be:  (140-540) with a healthy RT3 being around 165 or less, or (170-450) with a healthy RT3 being around 196 or less.

TSH: We have noticed that the best way to use the TSH lab test is in diagnosing a pituitary problem, not a thyroid problem. A very low TSH with a low free T3 gives away a hypopituitary issue. We do know that healthy people tend to have a TSH in 1’s and not much higher. We also know that many with clearly hypothyroidism can have a “normal” result sadly which will take years to rise high enough to reveal one’s hypothyroidism. Otherwise, we honestly pay no attention to it.

Supposedly, it measures the actual TSH in your body, called the Thyroid Stimulating Hormone, a pituitary hormone messenger. Yup, they are using a pituitary hormone to tell you if you have a thyroid issue. For example, if your body needs more thyroid hormones, the pituitary gland releases the TSH in order to knock on the door of your thyroid to produce more hormones. So theoretically, the higher the TSH lab test, the more your body is screaming at your thyroid to produce! produce! Creators of the TSH lab test came up with a ‘range’ that supposedly corresponds with healthy thyroid function.  So theoretically, if your TSH lab results are higher than the range, it would imply something is triggering your actual TSH to be a little too active in screaming at your thyroid. That something would be a diseased thyroid, called hypothyroid. But there are problems with this method of diagnosis. First, you can have a so-called normal result, yet be clearly hypothyroid with symptoms. Why? Because the TSH test cannot measure if all your cells & tissue are receiving the released thyroid hormones. Some may be (thus the normal TSH result) and some may not be (thus your clear symptoms). Second, if you have Hashimoto’s, you lab results can swing between hypo and hyper, & your lab test may be representing the middle of the swing.

T7, TOTAL T3, TOTAL T4, UPTAKE, etc: useless for our particular needs….

HASHIMOTO’S RELATED–two antibodies at the minimum

AB (Anti-thyroglobulin): Measures the level of the antibody protein anti-thyroglobulin in order to discern the presence of Hashimoto’s disease. Generally, if this is above the range, you’ve got the autoimmune thyroid disease Hashi’s.  It the result is below the “less than” mark, or in the range provided, you may be fine, but you need to do the anti-TPO shown below, as well. (No, we have not observed that it has to be zero to be free of having Hashi’s!)

THYROID PEROXIDASE ANTIBODY (ANTI-TPO): Measures the thyroid antibody TPO, which will be above the normal level in the presence of Hashimoto’s disease. Generally, if this is above the range, you’ve got the autoimmune thyroid disease Hashi’s.  If the result is below the “less than” mark, or in the range provided, you may be fine, but you need to have done the other antibody test as well–the AB shown above.

IMPORTANT NOTE:  since some with Hashi’s can also have the Graves antibodies, many patients are also testing both TSI and TRAB. TSI stands for Thyroid-Stimulating Immunoglobulin and TRAB stands for Thyrotropin receptor antibodies. TSI 80 or below is considered remission. Ideally, you should have zero TSI. Labs use either >140 or >125 (depends on the lab) as positive for Graves Disease. TrAb should be “undetectable”. Remission is when TrAb (PLUS the above TSI under 80) is less than .9. They also use a test called the TBII but we’re not sure what the perfect levels are for that one yet.

IRON RELATED (and you need all four, NOT just ferritin)

SERUM IRON (also called just Iron or Total iron): Measures the small amount of your circulating iron which is bound by the transferrin. You are looking for ‘close to’ 110 for women (or 109, or 108, or….), upper 130’s for men, based on what we’ve seen on hundreds of lab results. European or Australian lab ranges are something like this 7-27, and optimal is in the lower-to-mid 20s at the least for women and higher for men. If you are considerably higher than optimal, you could have the MTHFR mutation which will need testing and treatment. The MTHFR mutation also drives the ferritin low with normal or high iron is many of us, we’ve noted.  If all three iron labs are high (serum iron, % saturation, and ferritin, you may have the genetic hemochromatosis and you can ask your doctor for testing for that.

PERCENT % SATURATION of IRON: Measures your serum iron divided by your TIBC. When iron is good, women tend to be “close to” 35% (or .35 for Canadian ranges), we have discovered, and men go from 38% to 40-45%. Like all iron labs, you should be off all iron for at least 12 hours before testing to see how your supplementation is doing, or up to 5 days to see what your natural levels are. The latter may be best. NOTE: % Saturation can look falsely good or high if your TIBC is too low!!

TIBC (Total iron binding capacity): measures whether a protein called transferrin, produced by the liver, is enough to carry iron in the blood. Used to determine anemia or low body iron. If your result is high in the range and in the absence of chronic disease, you may be anemic. When iron is optimal as explained above, TIBC will tend to be in the low 300’s (with a range of 250 – 450) or for other ranges, about 1/4th above the bottom number in the range provided. NOTE we do NOT treat the TIBC. We treat the iron and % Sat. The TIBC just gives us interesting information as explained. 

FERRITIN: Measures your levels of storage iron. NOTE THAT WE DO NOT TREAT the FERRITIN LEVEL. A mistake. We treat iron and % saturation and let ferritin follow in its own accord. But ferritin is interesting to watch, and can also point to INFLAMMATION. Optimally, females often are around 70-90 with ferritin (Janie’s is 80 or less when her iron is good), though getting up to the 50’s has been good, too, when iron and % sat are OPTIMAL. Men tend to be slightly above 100, such as 110 – 120. 

If your ferritin is low along with inadequate/lower levels of iron and % saturation, that usually points to simply low iron, which is common with those on T4-only meds, or undiagnosed, or under-treated. But we do NOT treat that low ferritin. We treat the inadequate iron and % saturation, and over time, the ferritin moves up by itself if it’s too low.

If your ferritin is low with very good or high iron, plus a TIBC in the middle 300’s or higher, that usually points to having high heavy metals and an active MTHFR mutation.

If your ferritin is much higher along with less than optimal iron, it can point to INFLAMMATION, i.e. inflammation causes iron to be thrust into storage, and inflammation is common with certain thyroid patients for a variety of reasons. In less common cases, higher ferritin can be from liver disease, alcoholism, diabetes, asthma, or some types of cancer. But for most of us, it’s just about inflammation from hypothyroidism, or gluten issues, or unknown. So we need to lower the inflammation before taking iron supplements.

If ferritin is high along with a high % Sat and Serum iron, you may have hemochromatosis, an inherited condition. Time to get tested in working with your doctor.

By the way, we learned that we should be off all iron supplementation for at least 12 hours before testing to see what supplementation is doing for us, but 5 days to see your true iron levels.

FEMALE HORMONES (serum is recommended over saliva)

Progesterone… cycling women
 20-22 ng/mL (US) serum
64-70 nmol/L(UK) serum
250-300 pg/mL (US) saliva
1100-1300 pmol/L (UK) saliva
Progesterone… noncycling women
8-10 ng/mL (US) serum
25-32 nmol/L (UK) serum
100-125 pg/mL (US) saliva
440-585 pmol/L (UK) saliva
Estradiol… cycling women with normal SHBG
80-100 pg/mL (US) serum
294-367 pmol/L (UK) serum
1.30-1.50 pg/mL (US) saliva.
3.70-6.50 pmol/L (UK) saliva
Estradiol… noncycling women with normal SHBG
20-40 pg/mL (US) serum
73-147 pmol/L (UK) serum
0.40-0.60 pg/mL (US) saliva
1.50-3.00 pmol/L (UK) saliva
***NOTE: Women with high SHBG can have slightly higher estradiol. i.e. when SHBG is high (>160 or so), some need a level of 150-160 blood to feel well. As a noncycling woman with higher SHBG, some might need a level of 50-80.
SHBG is mentioned because so many women on T3/NDT/testosterone treatment have high SHBG.

FSH and LH for cycling women should be 1:1 ratio. If LH is higher, that typically means PCOS. Labs must be taken day 2-4 of the cycle while bleeding.

FSH/LH <10 mIU/mL good/healthy egg reserve (nowhere close to meno–chance of conception, <3 excellent, 3-6 good, 6-9 fair)

FSH/LH 10-15 conception difficult but not impossible
FSH/LH 15-20 perimenopause (probably not ovulating every month)
FSH/LH 20-30 menopause almost certainly in progress (ovulation rare if at all regardless of bleeding)
FSH/LH > 30 noncycling/postmenopause
Testosterone… both cycling and noncycling women
Free testosterone
2.10-3.20 pg/mL (US) serum
7.30-11.00 pmol/L (UK) serum
108-149 pmol/L (UK) saliva
36-47 pg/mL (US) saliva.
0.04-0.05 nmol/L serum
1.10-1.50 ng/dL serum
Total testosterone
28-38 ng/dL (US) serum
1.00-1.32 nmol/L (UK) serum
36-47 pg/mL (US) saliva
108-149 pmol/L (UK) saliva
 SHBG… cycling and noncycling women (and men) 75-95 nmol/L
DHEAs (in the absence of adrenal fatigue or PCOS): Serum 175-225 ug/dL Saliva > 13ng/mL


B-12: Measures an essential vitamin, B12, which can be low in hypothyroid patients due to low stomach acid. We noticed repeatedly that an optimal B12 lab result is in the upper part of the range, such as the upper quarter at least. It is NOT optimal to simply be “in range”.  For example, if your range is similar to 180-900, a healthy level appears to be 800 or higher. In the 500-800 range, you can benefit from taking B12 lozenges, specifically Methylcobalamin. The exception to the latter for some may be if they have both an MTHFR and COMT mutation–the methyl version of B12 can sometimes send out B12 levels way too high.

It has been shown in studies that patients with labs under 350 are likely to have symptoms, which means the deficiency is very serious and has gone on for a few years undetected. Lab ranges are much too low for B12…in Japan the bottom of the range is 500. The urine test Urinary Methylmalonic Acid, also called the UMMA,  can be added since it is a very sensitive detection and if high, will reveal a true B12 deficiency.

FOLATE: Also sometimes called “folic acid”, this is a b-vitamin which can be low in hypothyroid patients. Folate is important for prenatal development, as well as your blood cell health.  Folate works with B12 in the use and creation of proteins. It’s “folate” thats needed instead of “folic acid”, especially if you have MTHFR. We don’t start too high, as for some of us, it can start the methylation process too strongly. Standard range is 3-17, so optimal would be at least the top third of that, we have noted. Higher for MTHFR.

LIVER: The common tests we use are ALT and AST, and healthy, non-stressed livers always seem to put those tests in the teens, Janie has noticed over the years with patients.

MAGNESIUM: Thyroid patients can be chronically low in the electrolyte magnesium, which causes a multitude of problems ranging from worsened Mitral Valve Prolapse, less cancer protection, poor muscle development, too much calcium, cramping, and many other chronic conditions.  See Janie’s blog post on magnesium.  For RBC Magnesium, you are looking for an RBC result mid-range or higher, we’ve noted.

SODIUM (can also be strongly related to your adrenals and aldosterone): Measures the levels of the electrolyte sodium, which is outside cells, and has a balance with potassium, which is within cells. Sodium regulates bodily fluid and plays role in major bodily functions. This can be strongly related to whether you have low aldosterone or not. See Adrenals above.  We’ve noted that healthy folks will have this “close to” 142. 

POTASSIUM (can also be related to your adrenals): Measures the electrolyte mineral Potassium, which is within cells, and has a balance with sodium, which is outside cells.  Potassium plays a role in healthy kidney, heart and nervous system function. When potassium is too high, it’s called hyperkalemia; when too low, hypokalemia. It can rise in the presence of low aldosterone (see above under Adrenals), then fall. Best to do an RBC potassium–red blood cell—which measures it in your cells. We’ve noted that patients with healthy levels can be 4.2 and higher. If you do the RBC Potassium, our best knowledge shows it needs to be up in the 70-95% of the range.  Tell the lab tech NOT to use the tourniquet for drawing blood. It can falsely raise your potassium result.

RENIN: Measures the enzyme hormone that regulates the release of aldosterone and is done in conjunction with the aldosterone test. If renin is high in the range along with a low aldosterone, you have an adrenal cause. If both hormones are low in the range, you ‘may’ a pituitary problem. See this study to explain the latter. Always tested along with Aldosterone to see if your problem is due to the adrenals (primary adrenal insufficiency) or your pituitary (secondary adrenal insufficiency).

VITAMIN D test: (25-hydroxy) which is the most common Vitamin D test. Vitamin D plays a role in your immune system and other important actions. 50-80 is the most mentioned goal. Many thyroid patients are low in D due to digestive issues from being undiagnosed or undertreated, plus problems with Celiac or gluten intolerance.

A second Vit. D test you should ask for: Vitamin D1,25, the biologically active form of vitamin D. Why? You could have a genetic problem called the VDR mutation, i.e. the Vitamin D25 Receptor Mutation. Janie Bowthorpe has this mutation. See //www.stopthethyroidmadness.com/why-you-may-need-another-vitamin-d-test/

When someone overreacts to Vit. D supplementation, it seems to point to a parathyroid problem

ZINC: Top third of the range is optimal



Serum Iron (range) Optimal results are usually in the mid-20’s for women, upper 20’s and higher for men)

Percent Saturation:  same as US observations i.e. .35/35% for women is the ideal; .38/38% and often a little higher for men.

TIBC: when range is umol/L >45-77, low 60’s is noted when iron is looking good. If range is 50-70 umol/L, usually 1/4th above bottom of range.

Ferritin: range is often 15-200, and optimal for most women is 70-90, for men it’s 110-120.

Note: 60% of patients have a hematologic or neurologic response to B12 supplementation at a level <148 pmol/L

FOR THOSE WHO USE MATH–here’s how to find those “areas” of of lab ranges for thyroid, adrenals and TIBC

Top 1/4 of the range ** Math to do this, subtract the highest number in range from lowest number in range, divide by 4. Subtract this number to the highest number in range

Mid range ** Add lowest number in range to highest number in range. Divide by 2.

Bottom ¼ of the range: Subtract the right number of the range from the left number of the range, divide by 4. Add this number to the left number in the range




Want to order your own labwork?? STTM has created the right ones just for you to discuss with your doctor. Go here: https://sttm.mymedlab.com/

A great article about the fallacy of ranges: http://www.goodlifehealthcenter.com/2014/09/understanding-the-limitations-of-laboratory-reference-ranges/